Name: GSM7840816
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: All samples were stored at -80°C. Total RNA was extracted from 20 embryos per sample at the regenerative stage. Total RNA was extracted using TriReagent extraction and LiCl precipitation (Sigma) according to the manufacturer's instructions. The concentration of total RNA was determined using a spectrophotometer (Nanodrop 2000; ThermoFisher Scientific), and the quality of RNA was assessed using a Fragment Analyzer (Agilent, Standard Sensitivity RNA analysis kit, DNF-471). 100 ng of total RNA was used for library preparation using the Lexogen SENSE Total RNA-Seq Library Prep Kit. Library quality was tested by capillary electrophoresis on Fragment Analyzer (Agilent, NGS High Sensitivity kit DNF-474). The libraries were pooled and sequenced using Illumina NextSeq 500, 2x76 bp.